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Image Search Results
Journal: Scientific Reports
Article Title: A semi-automated, isolation-free, high-throughput SARS-CoV-2 reverse transcriptase (RT) loop-mediated isothermal amplification (LAMP) test
doi: 10.1038/s41598-021-00827-0
Figure Lengend Snippet: Composition of the SARS-CoV-2 RT-LAMP reaction mix. Deoxyuridine triphosphate (dUTP) and Antarctic thermolabile Uracil DNA glycosylase (UDG) were applied to prevent cross-over contamination. Additional WarmStart reverse transcriptase was added to increase reverse transcription efficiency. During the initial method establishment, a second reactions mix was set up per sample using a LAMP primer mix targeting the human beta actin gene (ACTB) instead of SARS-CoV-2 as an external control to account for sample quality.
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